Thought of the day...

Bismillahirrahmanirrahim....

Harini 240509...lagi beberapa hari lagi nak masuk bulan jun..sekejap je masa berlalu...bercakap tentang masa teringat kata2 senior sy, prinsip die dalam buat research ni jgn terlalu fikirkan masa...tapi fikirkan kerja yg kita buat. Walaupun tak dapat apa yg kita nak harini...cuba lagi, yg penting enjoy doing your research. Kalau terlalu fikirkan tentang masa kita akan sentiasa risau dan bila kita sentiasa risau,emosi tak stabil...then..buat apa pn tak jadi.


Inilah dunia research, tidak terlalu fikirkan tentang masa...bekerja tak kira siang ataupun malam, mengikut kajian yg sedang dijalankan. Kadang2 sibuk memanjang...kadang2 bersenang-lenang..Walau apapn kami mencari sesuatu yg membuatkan semua orang hidup gembira dan bahagia. Ayat-ayat ni sy ambil dari definisi Prof tentang bioteknologi sms inaugural lecture dia pd 8Mei lepas. Ayat nilah yg membangkitkan semangat saya..

Alhamdulillah.....sedikit sebanyak kata-kata diatas dapat memotivasikan sy harini....

Pagi tadi, trasa malas sangat nak ke lab, rs nak cuti harini. Tapi apakan daya, sample extraction belum siap sepenuhnya. Dengan keadaan tangan yg dah cramp (eh, betul ke eja ni?),dah lenguh sangat!...rasa tak mampu nak genggam micropipet tu lagi. Kalau boleh dgr suara tangan ni bercakap..mesti dia dah meraung2 kesakitan. Nasib baik semalam mintak tolong adik label appendorf microfuge tube yg banyak2 tu, kalau tak harini mesti dah surrender.Tapi kuatkan juga semangat untuk habiskan harini...dan bila dah start untuk 1st round centrifuge, some sample perform good, some sample perform bad...aduiii...sabar..sabar....Kira2 kalau macam ni, tak sempat juga siapkan harini, sbb harini sorang2,tak boleh balik lewat malam.


Blog nila tempat mencoret segala yg terlintas difikiran, teringat tajuk lagu Siti Nurhaliza: Diari Hatiku,..tapi blog ni bukan diari, macam diari je..hehe.

taken by my sister, yesterday...hehehe

My word is LOVE

Assalamualaikum.....

Wanna share u something!
I got this from one blog tt i follow---> KakChik's Wardrobe, she's very nice person,i like her style!,very fashionable and informative. I like her polyvore collection SO MUCH!....

Back to the topic, after take this fun and simple quiz, i got this.........

Your Word is "Love"

You see life as possibility to form deep connections with a few people. Relationships are the center of your world, and you always take time to bond with those you love. You are caring and giving. You enjoy helping those you love. And when it comes to romantic love, you feel passionately ... even in a very long term relationship.

What's" Your Word?

Try this!

it doesnt take long time, a few minutes only,

and Its Fun!

Protein solubility

Bismillahirrahmanirrahim.....

Wanna share with u something, mayb its usefull for your research? or may be not...just read...

Why are protein more soluble in base than in acid?


There are very many different kinds of proteins: some proteins precipitate in acid, and some precipitate in base. There are even proteins which do not precipitate when they are denatured. Therefore, it is difficult to answer this question for every case because of the large variety of proteins. Generally..................................

• Native proteins generally have highly folded structures.
• Heat or extreme pH cause this compact structure to lose its shape. This phenomenon is called “denaturation”. It will return to the native structure if the condition is quickly changed back to normal. However, if the condition is not changed for a certain time, it will not be reversible, because of aggregation and chemical reaction.
• Because the structure of a denatured protein is loose, hydrophobic parts, which were deeply buried in the native protein, will also encounter the solvent (water). It is energetically unstable when the hydrophobic parts contact water, as a result, denatured proteins aggregate with each other in order to prevent the hydrophobic parts from contacting with water. Therefore, denatured proteins usually precipitate because of their aggregation.
• Precipitation of denatured proteins increases because the solubility of aggregated proteins in water is usually low. For example, it is reported that a protein called “amyloid precursor protein” causes Alzheimer disease when it is denatured and deposited around nerve cells. Prion (infectious) proteins, known as causative proteins (protin penyebab) for the mad cow disease, also cause the disease because of their denaturation and precipitation. Once denatured prion proteins precipitate, they become “cores” into which normal prion proteins incorporate. Therefore, the denatured prion proteins are said to be infectious.
• The solubility of proteins is lowest at the isoelectric point (pI). Proteins have ionizable groups such as carboxyl groups and amino groups. Since the charge of these groups depends on pH, a protein molecule can have different charges according to pH. The number of negative charges is the same as the number of positive charges at the isoelectric point, therefore, the electrostatic repulsion between proteins is smallest at this point and the solubility is also lowest. “Isoelectric precipitation” is a process in which proteins are precipitated at pH close to their isoelectric point. This characteristic is applied to the crystallization of proteins.
• A protein whose isoelectric point is acidic is called an “acidic protein”. A protein whose isoelectric point is basic is called a “basic protein”. (My protein is acidic protein: because the pI is between 5.6-6.4)
• There are more acidic proteins than basic proteins.
• In general, positive charges and negative charges on the surface of protein are well-balanced around neutral pH. Because of electrostatic attraction, the shape of the protein is compact and stable. However, for example, at extremely low pH (acidic), the carboxyl group is protonated and negative charges are decreased. Thus, proteins will lose the stability which comes from electrostatic attraction, and gain more electrostatic repulsion between the increased positive charges. This is how proteins are denatured at extreme pH.
• When acidic proteins denature in an acidic condition (for example, pH 2 – 3), proteins aggregate with each other easily and the precipitation increases at the pH near the isoelectric point (where electrostatic repulsion is low). On the other hand, when basic proteins are denatured in acidic conditions, they do not aggregate very much because the proteins have many positive charges in the acidic condition and electrostatic repulsion is high. Therefore, when the pH is brought back to neutral, non-precipitated basic proteins will return to their native structure, but this is not generally true for precipitated acidic proteins. However, the return of acidic proteins is possible when the proteins are dissolved in a very thick denaturant (urea or guanidine hydrochloride, for example) solution, followed by treatment with physiological conditions.
• The concept is essentially the same for basic conditions as for acidic conditions. However, with a relatively long treatment, acidic proteins will precipitate even in basic conditions, since peptide bonds are broken and sulfur is removed by the excess hydroxide ions in basic solutions. Take eggs as an example. Although the egg white is weakly basic (pH 9.2), the damage to proteins is as severe as under strong basic conditions when an egg is heated (picture: boiled egg). Even ovalbumin (acidic protein, isoelectric point: 4.6), which composes 75% of the proteins in egg white, will precipitate. You can smell H2S gas (volcano) from hard-boiled eggs. This is the evidence for the removal of sulfur from proteins. In conclusion, basic conditions are not usually employed for the treatment of proteins, since proteins are severely damaged in basic conditions.

• Since there are more acidic proteins than basic proteins, more proteins are precipitated in acid. However, this is only the short term case. In the long term case, proteins can precipitate in basic solution as well. The difference is that precipitated proteins in acidic solutions are usually not damaged, and can return back to their native structures, but, precipitated proteins in basic solutions are usually too damaged to return.

OK.....then i know a bit things about the problem of my protein ----> insoluble @ aggregates in the cells. My protein is acidic protein but the pI is near to neutral. If the pH in the cytoplasm changes, even 1 point, it may effect the protein solubility.....hmmm.....another thing is, phosphate buffer which is used in the media is the most uneffective kosmotropic salts (mean=makes the aggregation or precipitation occur). This another thing need to be considered!..if i change the buffer system, E.coli may not accept the new buffer system...(-_-)...aduiii.....

But this is not the only thing to be considered, i need an information about the mechanism how and why the E.coli making such aggregates??? for food during starving time? for energy? just assemble the rubbish?...i dont think so....

what do u think?

Acknowledgement:

Thank u to Dr. Hidenori Yamada for providing the brief answer, n special thanks to www.chemistryquestion.com, wikipedia.com

=)

Never giveup!

Bismillahirrahmanirrahim......

Holiday in the lab again!....

Just thought want to drop something in the blog. As usual, when im doing lab work,short time while waiting for next sampling or centrifuging sample, i will simply click on anything what is passing in my mind..then search. These few days, after rolling my mind up and down searching for journals, ideas, facts etc..etc..etc for lab report...lastly endup with fashion! =P..hehehe...

I dont know why this interesting topic attract me so much during the peak of my RSM report..."sambil menyelam minum air"...huhu!.....what im going to say,i found a lot shopping boutique online!!..huhuhu

I cant finished the report rite now, cause need some data to complete it, thats why im here...when other people hanging out with friends and family; i? repeat experiment.... (T_T)...if my bacteria grew up yesterday, very sure today i will not be here.But, this time i dont feel too bad, since long time i dont do labwork. Just regret for a few hours,then...planning to grow new batch. Alhamdulillah....the second batch grew happily.!!

Nothing happy without seeing u grow.....

"Thanks God"

-Picture shows my Rosetta in buffle flask, which ready to be extracted

-from these, i will get my protein...\(^0^)/...huhu

((150509-update on 190509))

Jom jalan-jalan

Assalamualaikum....

Today, just wanna share with u the pictures we took at "Naval Town"...Lumut,Perak (on 25-26th April 2009)....

Saturday 250409

05.00 pm A journey beginnn.......

10.30 pm Arrived Manjung (Rest house already booked by friend on friend member)

Sunday 260409

09.00 am Keseronokan bermula.....huhu!

Our first destination is Kompleks Sukan TLDM. Kat sini kitorang nak tengok rehearsal untuk perbarisan esok. disebabkan esok kitorang dah nak balik.....masing2 pn keje n ada exam + labwork, so dapat tengok diorang rehearsal pn jadila....saje cuci2 mate..hikhik!

okey....masing-masing ambil tempat duduk masing-masing......

Sebenarnya, masa kitorang sampai rehearsal dah mula lama dah....so,sampai2 tu layan jela....hmm...sungguh kagum!.Sambil2 tengok yg berkawad, tengok skali gelagat navy2 yg ada d sekeliling track tu, kelaka pn ada...pegawai2 yg bertugas sibuk cek kedudukan badan, tangan, betulkan topi, betulkan senapang...macam-macam lagila...kalau sy kat tempat diorang tu...rasa naik rimas gak,semua tak kena. Tapi apa pn itulah yg terbaik untuk diorang, kalau tak, tak nama askar kalau bukan mcm tu...betul x?

Dah puas tengok rehearsal perbarisan, terasa lapar pulak....sbb tak jamah2 apa2 pn masa kat resthouse tu. Kitorang makan2 kat food court dalam kem ni.....apa nama die...emmm..tak ingat dah...(short term memory...he2).

Perjalanan diteruskan.....kalini, kitorang nak melawat kapal2 tentera yang gagah perkasa!!!(bukan kapal terbang ye) ....jom!

Perasaan berkobar2 nak naik kapal ni....

Ada banyak kapal kat sini....tak ingat ada berapa banyak dan setiap kapal tu ada function tersendiri....Setiap kapal ada KD kat depan..tau tak apa KD tu? KD tu "kapal diraja". agak lucu jugak...sbb banyak kali saya tanya soalan yg sama, sampaikan sepanjang perjalanan fikiran sy dok hafal KD tu kapal diraja....yela, taknak kena perli lagi...hihi =p

Ni kat atas KD Kinabalu......

Posing lagi......huhu!!!

Bergambar sebelum naik kapal perang terbesar ni..kapal ni dah banyak kali menang anugerah, n paling banyak berjasa...nama dia KD Sri Inderapura.. =), lepas navy day ni...KD ni akan belayar ke Somalia untuk misi keamanan...cam gitula.....semoga anak2 kapal beserta kapal selamat pergi dan pulang...amin...

Jom!.....naik tinggi lagi

Cari muka pegawai2 dikenali nih =P.....beria ina & aida

Kat belakang tu.....kapal Fajar Samudera....kapal untuk pelancong,tak dapat nak terangkan lebih2 sbb tak sure sgt. Banyak sangat kapal kat sini....tak sempat nak naik semua. Cuaca pn elok sangat, panas terik tak terkata.

Okey, dah penat dan lapar......cukupla setakat ni....KD2 yg tak sempat explore tu, kita postpone tahun depan, insyaAllah....

Keep Walking....

The Organs of your body have their sensory touches at the bottom of your foot, if you massage these points you will find relief from aches and pains as you can see the heart is on the left foot.

Typically they are shown as points and arrows to show which organ it connects to. It is indeed correct since the nerves connected to these organs terminate here. This is covered in great details in Acupressure studies or textbooks. God created our body so well that he thought of even this. He made us walk so that we will always be pressing these pressure points and thus keeping these organs activated at all times.

So, keep walking...

Thought of the day...

Bismillahirrahmanirrahim......

Busannye.....dok kat lab sensorg nih....slalunye ade je bdk2 ni dtg,walaupun tak wat keje lab...ermm...mungkin betul2 cuti untuk cuti hari pekerja ni.kalau ikutkan hati mmg nak cuti gak...yela macam2 plan nak wat cuti 3hari ni,tapi dsebabkan ada sample tak extract lagi...terpaksala memaksa diri untuk dtg lab...kalau tak dipaksa...alamat selamat bubbye la sample tu....ermm....takleh2!...dahla fermentation kalini menjadi..fuh! dah lama menunggu saat2 dia sihat dan kuat,akhirnya....berjaya!,takkan nak bazir sample tu kan?.

Itula lumrah hidup ni..ada masa kita sihat...ada masa kita sakit, kdg2 segala yg kita buat berjalan lancar,kdg2 setiap langkah tersungkur...ada je halangan....yg penting kena doa banyak2 supaya apa yg kita buat mendapat keredhaanNya.... Pesanan yg saya dapat satu hari tu....kita ni sentiasa kena berdoa..supaya jgnla apa yg kita buat, lari dari landasan yg telah Allah tentukan untuk kita...bukan akidah je kita kena jaga supaya tak terpesong dari landasanNya, tapi soal kerja harian pun same gak. Sebab tu kadang2 apa yg kita buat asyik tak menjadi....tak seperti apa yg kita harapkan...mungkin ada yg tersilap. Mmg itu kdg2 kita kata itu adalah ujian...betul...tapi apa tujuan Allah bagi kita ujian? sebab...supaya kita kembali kepadaNya, mengingatiNya, dan tak lari jauh dari landasanNya....kalau dah lari jauh,tak ke susah nak patah balik?...hmmm.....terima kasih kepada pemberi pesanan & semangat....terima kasih Kak Dayah!! terima kasih!.. =)

Satu lagi, jangan lupa doakan kawan2 kita selain doa kpd mak ayah (doa kpd mak ayah tu mmg wajib)....kadang2 kita lupa untuk doakan org2 sekeliling kita. Bila kita dalam kesusahan,kemurungan,kebuntuan...etc..etc..kita slalu berdoa supaya Allah bantu kita, bukakan jalan untuk kita, segala2 nya untuk kita,tapi....sedarkah kita bahawa kadang2 pertolongan Allah tu datang dari org2 sekeliling kita? Allah tau dalam sesetengah perkara mmg kita tak mampu nak buat, then...Allah datangkan pertolongan kepada kita melalui org2 sekeliling kita yg lebih mampu. so...jgn lupa doakan org2 sekeliling kita =).

Hmm...bagi motivasi sket..hehe...