why e.coli form inclusion bodies??....for what purpose or the formation is due to what condition???.....
In industrial production of biopharmaceutical products,this things is the major problem...when higher expression = overexpression of recombinant gene was achieved, this inclusion bodies is start produced by the host especially in bacteria (eg e.coli)...and this will effect in the downstream process where additional process are required....
actually what happen inside the e.coli?
please give me the answer...(ask my self....)
there is a case where...one biopharmaceutical protein was inserted into one e.coli strain....after several fermentation process still cannot make a conclusion about the growth profile and still cannot reach the expected OD....i think this is not due to the fermentation condition...but all come from the basic mechanism inside the e.coli.....
even this microorganism had been studied in many research area....but people just force them to produce sumthing they want but...not consider what is needed by this poor microorganism....poor e.coli =(
How can i extract the knowledge about it??.....i need a strength frm deep inside my self....
Ya Allah...bantulah hambamu ini mengkaji ciptaan mu Ya Allah....
deep inside......i want to finish up this.....and start new one....... =(
Qber oh Qber...
Tuesday, March 24, 2009
Rindunye kat sekola lama!!!!.....
Qber oh Qber!....
Harini buka frenster je trus nampak gamba pondok kat sebelah bilik lama kat Qbe dulu...Waaa!!! tak sangka dah banyak perubahan,siap ada bangunan tinggi2 lagi,kalau tak dulu macam umah panjang je..da la renek2 je. Walaupun byk kenangan pahit kat ctu tapi betulla kata org walau mcmmana buruk pn tempat asal kite, satu hari ia akan menjadi satu kenangan yg indah buat kita. Masih ingat lagi, 7 tahun lepas....ms nak ambik keputusan SPM, nak jumpa cikgu pn susah..masing2 bz..ada la gak rs terkilan..ish, mmg takleh lupala.tapi tahun demi tahun berlalu...yg buruk2 tu lenyap diganti dgn kenangan yg indah2 saja....betulla bak kata pepatah "hujan emas dinegeri orang, hujan batu di negeri sendiri...lebih baik di negeri sendiri.."
my favourite...may be u?
Monday, March 23, 2009
hari ni mmg taknak wat keje.....dah start extract protien, takat periplasmic je..pk2 balik, not worth kalau nak teruskan,so i biar je sample tu dalam centrifuge tu...huhu..skrg ni mood nak tulis pasal my favourite and maybe u?
belum tulis lagi dah tersenyum lebar...first question ----> what is ur favourite cartoon???
banyak kan? and the latest i want to share with u all is this cutest hero for kids ----------------------->
belum tulis lagi dah tersenyum lebar...first question ----> what is ur favourite cartoon???
banyak kan? and the latest i want to share with u all is this cutest hero for kids ----------------------->
Wonder Pets!!!.... trade mark die "apa yg penting? kerjasama!!"..saya percaya mesti sape2 pn akan terpikat dengan ke"kiutan" dan kecomelan cartoon ni.dan lagu dia yg mak saya kata "takde pitching langsung" tu.. telah manjadi siulan bukan sahaja kanak2 malah org2 tua pun...saya pn hafal jugak lagu die!...
Wonderpets ni cartoon UK..kalau tak silap saya.ia cartoon yg sgt simple n mudah difahami, penuh dgn nilai2 murni..esp suka tolong binatang dalam kesusahan...daripada lirik die:
"kami tak besar dan kami tak kuat,
bila bekerjasama..semua jadi mudah...
heyyyy......wonderpets!!"
wonderpets ni terdiri daripada 3sekawan binatang yg hidup dalam nurseri@tadika... -->ling, tuck and ming-ming...misi pertolongan cemas mereka bermula bila ada telefon berbunyi....then diorang akan nyanyi "..telefooon....telefon berbunyi....." Then..bila misi dapat diselesaikan mereka akan raikan dgn daun saderi@ cellery (xtau nak eja)..haha...comel sgt!
Cartoon seterusnya.....
Oggy and the cockroaches!!!
Dah lama tak tgk cartoon ni..dulu tv siarkan pukul 10 pg,tp skrg pkul 8...slalu miss..hehe...sbb bgn lambat..ups!..hehehe....cartoon ni pn best walaupun kadang2 menyakitkan hati...kesian kat oggy, slalu kene buli dgn cockroaches tiga ekor ni. mggu lepas miss tgk cartoon ni, tp tak kisah sbb ada siaran ulangan, tapi bukan kat TV tapi my mom yg akan cerita balik apa yg berlaku kat oggy haritu..huhu!!! besh2!!...untuk yg mggu lepas dalam aksi kejar mengejar oggy dgn lipas2 ni..oggy dapat tangkap salah sekor lipas ni...masa oggy tangkap, bila die nak pukul je...si lipas yg malang tu pn buat muka kesian n isap jari..(wat mcm gaya baby)...oggy yg berhati mulia ni pn kesian...n tak jadi nak pukul n penyek lipas ni...Then, oggy layan macam anak dia...pakaikan baju mcm die...letak lipas tu kat buaian...(mcm baby)...ajar die gosok gigi..etc. Satu hari masa oggy nak tutup pintu fridge, tiba2 lipas tu tersepit kat pintu fridge tu, lipas tu pun marah sangat kat oggy,dia joint semula kwn2 dia yg jahat tu....dan bermulala....kerja2 jahat diorg membuli oggy...
Satu hari,masa these cockroaches mengintai oggy kat bilik air...lipas yg pnah baik ngn oggy ni ternampak tempat die n brg2 (berus gigi..ubat gigi...etc)...n terkenangkan masa oggy ajar dia gosok gigi...isk..isk...sedih =(..bila kawan2 dia ajak dia nak kerjakan si oggy ni dia taknak....dia sedih......commel sgt!....okey,sampai cni je mak cerita....
Third cartoon is......PINGU!
cartoon ni pun comel!..cerita ttg kehidupan seharian pingu&famili....perangai die macam budak2 kat malaysia...kadang2 baik...jaga adik,tolong mak kemas rumah...etc..kadang2 nakal, suka kenakan org,suka kacau adik...merajuk....tapi akhirnya bila di pujuk dia akan baik semula..hihi...so cute! u must watch this series.
sekadar coretan
bismillah...
baru terasa yg sebenarnya hari2 yg dilalui tak berapa indah,kalau nak dbandingkan dgn org lain,keje mmg keje..tapi taklah seteruk kehidupan aku ni yg ntah pape...org kata takde life...asyik2 kat lab je.bosan betul!
tapi....kalau difikirkan balik, ada sbb saya jadi camni.sbb apa? sbb nak mengejar kehidupan yg senang dkemudian hari...insyaallah...kalau diizinknNya...itulah yg hari2 diimpikan.Bersusah2 dahulu...kalau diikutkan hati mmg dah bosan tahap gunung berapi yg nak meletup dah!(hanya org2 tertentu sahaja yg faham keadaan ni)
So, untuk memujuk hati ni....cuba fikirkan ttg kesusahan org2 yg susah,yg mmg susah..slalu ditimpa nasib malang walaupun si empunya diri dah tak mampu lagi nak menanggung derita.ish, kesiannya...Allah maha adil,terimalah diri kita seadanya
baru terasa yg sebenarnya hari2 yg dilalui tak berapa indah,kalau nak dbandingkan dgn org lain,keje mmg keje..tapi taklah seteruk kehidupan aku ni yg ntah pape...org kata takde life...asyik2 kat lab je.bosan betul!
tapi....kalau difikirkan balik, ada sbb saya jadi camni.sbb apa? sbb nak mengejar kehidupan yg senang dkemudian hari...insyaallah...kalau diizinknNya...itulah yg hari2 diimpikan.Bersusah2 dahulu...kalau diikutkan hati mmg dah bosan tahap gunung berapi yg nak meletup dah!(hanya org2 tertentu sahaja yg faham keadaan ni)
So, untuk memujuk hati ni....cuba fikirkan ttg kesusahan org2 yg susah,yg mmg susah..slalu ditimpa nasib malang walaupun si empunya diri dah tak mampu lagi nak menanggung derita.ish, kesiannya...Allah maha adil,terimalah diri kita seadanya
Selamat Berjuang!!
Monday, March 16, 2009
long time not update.....
after release from dmm, i bcome workaholic
kije..kije..kije....
think! think! .....thought!
May Allah bless what we do and bring us shining star to shine our future!!
waiting for miracle.... (^_^)V
Matric Kuala Pilah in Memories....
Tuesday, March 3, 2009
2306, Monday, 02 March, 2009.....
Study time!!!.....flash back to year 2004.. =), ..in the silent and calm night...it was struggling time...everybody was dreaming of 4flat,every day...especially when exam was coming soon. For me, i like to hear nasyid during study, cause it gives me an inspiration and bring me to a new world, that only me and books there...izza, my dear friend who makes me fall in love to inteam song....~nurkasih~sutera kasih~bisikan nurani~~~etc..etc..etc...while ada, i never never forget about her fanatic to haikal group untill we called her "ada haikal"..huhu!..besh2!...and now, it comes again!!... i just open real player ---> first song from In-team~~Kasihku Abadi~~...never forget that prep time in Matric Kuala Pilah....(^_^)V"..hihii...prep in our own style----> terbaring?? terduduk??...tertido??..what ever position u like….in any place...on your table,on the bed..in the classroom, anywhere!!...cool meh?..thats the cool matric,never have fix rule to prep, I like!!...hahaha…. (not sure this time,still same or not *o*??)
Now...the same time but 5 years after...i still in the mode of study, but different title..that time im still 19 years old...(huhu…sweet teen ages), still matric naive student (comot.. ;P) ..dont know what happen tomorrow and 2 years ahead.....and now!...the same person, same name...the only me...study again..but..with "postgraduate" title..master's graduate to be...insyaAllah. Subhanallah...& Alhamdulillah...creating somebody to be, Thanks God...because gave me a chance to experience all these precious moment which never one had,only me...(different people, different way), it such a great journey..
Now, this In-team song ..~~Sutera Kasih~~..still being my favourite song ever after...cause it brings me come back to my beloved matric----> Kuala Pilah, and all my beloved friend =)..miss u all...I miss my biology lecturer,Pn Abdiah...my math lecturer, Miss Rabiatul Adawiyah...all my practicum mate and Kuliyyah mate ----> 4Hayat ~(^0^)~...
Never forget my block mate-----> cendekia members =) especially 4th floor.. huda ,fiza,zie,fid,akma,za,ada,fatin,norriah, noma, angah...etc..etc...etc...miss u all...
Study time!!!.....flash back to year 2004.. =), ..in the silent and calm night...it was struggling time...everybody was dreaming of 4flat,every day...especially when exam was coming soon. For me, i like to hear nasyid during study, cause it gives me an inspiration and bring me to a new world, that only me and books there...izza, my dear friend who makes me fall in love to inteam song....~nurkasih~sutera kasih~bisikan nurani~~~etc..etc..etc...while ada, i never never forget about her fanatic to haikal group untill we called her "ada haikal"..huhu!..besh2!...and now, it comes again!!... i just open real player ---> first song from In-team~~Kasihku Abadi~~...never forget that prep time in Matric Kuala Pilah....(^_^)V"..hihii...prep in our own style----> terbaring?? terduduk??...tertido??..what ever position u like….in any place...on your table,on the bed..in the classroom, anywhere!!...cool meh?..thats the cool matric,never have fix rule to prep, I like!!...hahaha…. (not sure this time,still same or not *o*??)
Now...the same time but 5 years after...i still in the mode of study, but different title..that time im still 19 years old...(huhu…sweet teen ages), still matric naive student (comot.. ;P) ..dont know what happen tomorrow and 2 years ahead.....and now!...the same person, same name...the only me...study again..but..with "postgraduate" title..master's graduate to be...insyaAllah. Subhanallah...& Alhamdulillah...creating somebody to be, Thanks God...because gave me a chance to experience all these precious moment which never one had,only me...(different people, different way), it such a great journey..
Now, this In-team song ..~~Sutera Kasih~~..still being my favourite song ever after...cause it brings me come back to my beloved matric----> Kuala Pilah, and all my beloved friend =)..miss u all...I miss my biology lecturer,Pn Abdiah...my math lecturer, Miss Rabiatul Adawiyah...all my practicum mate and Kuliyyah mate ----> 4Hayat ~(^0^)~...
Never forget my block mate-----> cendekia members =) especially 4th floor.. huda ,fiza,zie,fid,akma,za,ada,fatin,norriah, noma, angah...etc..etc...etc...miss u all...
Usah putus asa
Monday, March 2, 2009
Ketahuilah olehmu sahabatku...
Jika kau merasa lelah dan tak berdaya dari usaha yang sepertinya sia-sia... Allah SWT tahu betapa keras engkau sudah berusaha.
Ketika kau sudah menangis sekian lama dan hatimu masih terasa pedihnya...Allah SWT sudahpun menghitung airmatamu.
Jika kau fikir bahawa hidupmu sedang menunggu sesuatu dan waktu terasa berlalu begitu sahaja ... Allah SWT sedang menunggu bersama denganmu.
Ketika kau merasa sendirian dan teman-temanmu terlalu sibuk untuk menghubungimu...Allah SWT selalu berada disampingmu.
Ketika kau fikir bahawa kau sudah mencuba segala-galanya dan tidak tahu hendak membuat keputusan ... Allah SWT punya jawapannya.
Ketika segala sesuatu menjadi tidak masuk akal dan kau mula merasa tertekan...Allah SWT dapat menenangkanmu.
Jika tiba-tiba kau dapat melihat jejak-jejak harapan...Allah SWT sedang berbisik kepadamu.
Ketika segala sesuatu berjalan lancar dan kau merasa ingin mengucapkan kesyukuran,Allah SWT telah memberkatimu.
Ketika sesuatu yang indah terjadi dan kau dipenuhi ketakjuban,Allah SWT telah tersenyum kepadamu.
Ketika kau memiliki tujuan untuk dipenuhi dan mimpi untuk digenapi,Allah SWT sudah membuka matamu dan memanggilmu dengan namamu.
Maka ingatlah..walau dimanapun, atau kemanapun kau menghadap...
Allah SWT TAHU
p/s: copy from i luv islam
Memories
aiseh....suddenly the memories in Q-ber and matrik Pilah appear..whoaa!!! miss u all!!!...miss so much!. cant imagine with words..(^0^)V"....i miss my roomate in Q-ber (sahada...ezzah...& hanis..), my block mate in matrik pilah (za, norriah, sahada again....huda (where r u??)...fiza..zie..fatin..noma..fid...all my neighbour) ..my study group mate (naf, wan)..my practicum mate...what r they doing now??..where r them??...this song make me missssss all the memories in Q-ber....
Q-ber...
I still remember when we r in form 4, our room is between senior room....every night before go to sleep, we always talk about our senior...they r like a crazy step mother!..ask the junior do this,do that..but they dont..i hate them..the most biggest thing that i cant forget untill today is about my "special senior" of senior-junior program (ntah pape ntah, takde pekdah langsung!). He is kind,nice and very caring senior..BUT at the same time he is a "hero" of fighting-fighting.(*o*)??confuse~~...i know about his real character from other girlsss senior. his name? R**i. His girlfriend...kak S**m... What i heard from other senior he is bad boy..even all the teacher in Q-ber know who is R**i. since their scandal being "terbongkar" i hate them. why it happen to me?? that time is very tough for me.....all the senior know who is the "lucky junior"...because of that issue, i became popular,...hahahaha!!. one day a senior (of coursela girl..) seems like want to fight with me (i will remember her face untill ever after...), i dont know what she want from me, i never did anything related to her and never have any relationship with her.because i am junior n "penakut", i just let it. Thanks God, that time still have senior want to protect me and care about me...Kak Nani (my neighbour- thank u kak nani =)) and kak keyon....
A lot of things happen during study in Q-ber, form 4 we have "floating class", I think this situation u will never find in other boarding school. we dont have our own class... among junior (form 4)..we need to share class room,every subject different classroom...very2 tired. A few month later....two rooms (Bahasa and IT) was opened to make our class.then no floating class again..alhamdulillah...
Shortened...List of some memories that i cant forget about Q-ber:
- Kisah arang & ikan (Coop week)...hahaha!!!....
- Tennis Court....misssss this....
- Outing
- Dusun pakcik ada
- Graduation day (^_^)
- my Homeroom =)..-----> homeroom day.hehehe... i got "request" chocolate from secret admire..ngeh..ngeh...ngeh...
- Camping at Sekayu waterfall
- Bio experiment
- PRS (especially during interview....huhuhu!!!)----> i'm the interviewer
- assembly (when our naqib sang this song ---> "puteriku sayang")...wink!!wink!!...hahaha cant imagine all girls....cair...wakaka
- etc..etc....etc....(u may add on if u're q-berian 01-02)
Matric Kuala Pilah.......to be continued....
(written by annie, 0017, Saturday 280209)
Q-ber...
I still remember when we r in form 4, our room is between senior room....every night before go to sleep, we always talk about our senior...they r like a crazy step mother!..ask the junior do this,do that..but they dont..i hate them..the most biggest thing that i cant forget untill today is about my "special senior" of senior-junior program (ntah pape ntah, takde pekdah langsung!). He is kind,nice and very caring senior..BUT at the same time he is a "hero" of fighting-fighting.(*o*)??confuse~~...i know about his real character from other girlsss senior. his name? R**i. His girlfriend...kak S**m... What i heard from other senior he is bad boy..even all the teacher in Q-ber know who is R**i. since their scandal being "terbongkar" i hate them. why it happen to me?? that time is very tough for me.....all the senior know who is the "lucky junior"...because of that issue, i became popular,...hahahaha!!. one day a senior (of coursela girl..) seems like want to fight with me (i will remember her face untill ever after...), i dont know what she want from me, i never did anything related to her and never have any relationship with her.because i am junior n "penakut", i just let it. Thanks God, that time still have senior want to protect me and care about me...Kak Nani (my neighbour- thank u kak nani =)) and kak keyon....
A lot of things happen during study in Q-ber, form 4 we have "floating class", I think this situation u will never find in other boarding school. we dont have our own class... among junior (form 4)..we need to share class room,every subject different classroom...very2 tired. A few month later....two rooms (Bahasa and IT) was opened to make our class.then no floating class again..alhamdulillah...
Shortened...List of some memories that i cant forget about Q-ber:
- Kisah arang & ikan (Coop week)...hahaha!!!....
- Tennis Court....misssss this....
- Outing
- Dusun pakcik ada
- Graduation day (^_^)
- my Homeroom =)..-----> homeroom day.hehehe... i got "request" chocolate from secret admire..ngeh..ngeh...ngeh...
- Camping at Sekayu waterfall
- Bio experiment
- PRS (especially during interview....huhuhu!!!)----> i'm the interviewer
- assembly (when our naqib sang this song ---> "puteriku sayang")...wink!!wink!!...hahaha cant imagine all girls....cair...wakaka
- etc..etc....etc....(u may add on if u're q-berian 01-02)
Matric Kuala Pilah.......to be continued....
(written by annie, 0017, Saturday 280209)
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My time in Sheffield, UK
