Im so happy!!

Assalamualaikum....

i'm so happy!!...that is what i feel after came back from Lumut. Even there's something wrong somewhere, at least i can forget all the project's problems for a while,and come back with new mood,...so refreshing!!"V(^0^)V"...

Another things that makes me happy is....when i open my emel, i got so many comment in my blog...waaaa!!!! so happy!!!...that's totally reflect the words ----> finally got my favour...huhu!. the most important thing is i got new friend ennacute and slurpee. thanks for visiting my blog. thank u to mama za too!...

I've sumthing to share ( about my trip to Lumut = Navy Day), but i didnt receive the picture i took at Lumut from my friend, ina. Ina...she's also my new friend, not forget....aida (driver) and izurin....thanks to wanna aka ejan, cause invite me to join this nice vacation n introduce me to ina,aida n izurin....miss u all!!....

(watching rehearsal for tomorrow's real "perbarisan"..hehe..)

Ok, i need to start my work now....

(hmmm....dah lama rsnya tak masuk lab,ok caiyo'2x! ani...do your best,pray for the rest)

Finally...got my favour

Alhamdulillah.....

After changing few templates for my blog, finally i got what i want..huh!satisfied!..Previous template was changed unconsciously, tak sedar tgh survey2 template baru....suddenly, terklik!..just one click,my blog change.then....again...need to get back all the link.

Another achievement for today are:
* successfully edit my 3D response surface for RSM, with favor (a.k.a.help) from collaborated software=Ps3,paint,powerpoint,excel,word(of course la..),RSM(this must!)
* successfully edit my intro....as my supervisor said..make it shorten as much as possible...(okey prof..)

Next target:
* plan for the next experiment. Now, im interested in physiological and biochemical studies,but how can i do a lot of things at one time? the most important question ---> how can i solve my protein problems?

As i go through this journey as postgraduate student, day by day i realise tt knowledge is priceless,a lot of interesting things u will get every day, even every minutes....just let your problems aside..flash back (or muhasabah),what u have done before..did u realise it?...think!think!think!...masyaAllah....no words could describe that feels. When u meet other people out of your field, even in the same field...how wonderful knowledge it is, and how big it is!...hmmmm....kerdilnya diri ini '(-_-)"'

May God bless us

wondering...."(~_~)??"

Bismillahirrahmanirrahim......

Hmmm.....just wondering//~~..why some researchers were successfull in producing high amount of IFN-gamma, but not alpha...whats the different between these two?,they r same glycoprotein, just differ in type.is it just due to the type make it totally different in the expression??....continue search.....search...search.....(~_~)""....hmm~~~,need this info to convince people

Anybody know?

Assalamualaikum........

Today is the hundreds time after i tried so many time to get the effect of different pH on my protein solubility..as was predicted by me and my supervisor...the solubility of my protien may increase when the pH during fermentation is far away from pI value of my protein. But....what was expected....just as an expectation...... "harapan tinggal harapan... =("....

From the first i tried to prepare the buffer for each of pH until the protein quantification........still dissappointed...How difficult to prepare the buffer (what i had faced before......waiting for the stock..looking for the chemical...searching for the methodology.....calculating using Handerson-hasselbach......)----> actually not as difficult as what had been happen to me. That remind me about "sign"....sign is situation in which when we want to do something, the starting job reflect to the final job or the results. that is what "org2 tua" said...when you feel something uncomfortable b4 doing something,dont proceed?...sometimes good to follow this =)....hmmm....out of topic.its ok, at least i can release all the blocking2 area in my mind..

Back to the topic, anybody can help me,give some idea to make my project succesfull...i want to see the effect of different pH on my protein solubility. At the same time looking for the pH that give higher solubility. im using commercial media...TB(terrific broth),in TB media, the buffer system is phosphate buffer (0.06M), i increased the buffering capacity into 0.2M at the same time adjust the pH from 6 to 8 at the starting point of induction. Before this, i tried to adjust the pH from the starting time of fermentation,but the result is very2 frustrated =(.... all the cells in prepared buffered-media dead!!!....sob!sob!..

Then...i tried using TB for all pH,then once induce,change the pH with buffer of different pH. Still...the growth of e.coli not as expected....very slow....compared to the control....Now, im very tired to do all these. The latest trial also, i dont have any intention to proceed with the next step of protein extraction.

Whar r your suggestion?? help me......

Ketahui rahsia penipuan dunia internet

Assalamualaikum......

Nak share something yg patut semua org tau,especially yg nak tambah pendapatan melalui program2 yg ditawarkan melalui internet. Sebenarnya, saya pn berminat nak buat duit melalui internet ni,tapi mcm ragu2 jugak.Dulu pernah skali mencuba ttg program affiliates,tapi tak menjadi sbb nak buat kerja2 macam tu memerlukan kita untuk duduk depan internet sepanjang masa,lagi2 kalau br nak mula.agak susah,tak macam apa yg dijanjikan. Untuk kali kedua sy ingin mencuba yg lain tapi kali ni saya berhati2 supaya tak melayang duit saya kali kedua walaupun cuma beberapa ringgit sahaja. Dan kalini saya ada terjumpa artikel ni.....ia membuatkan saya terkedu seketika...sekejap je,terus saya buat keputusan untuk sebarkan. Terpulang kepada anda untuk percaya atau tidak,saya hanya menyampaikan sahaja. artikel ni panjang, so sy tak tulis semula,saya link ke file yg saya dah download di bawah:

"alamak.....takleh nak display la plak..........."

nantila saya update balik

tapi kalau berminat nak baca artikel tu,emel saya --> neo_82001@yahoo.com

Aduh!....

bismillahirrahmanirrahim......

Penatnye nak kisah pasal problem ni...bila kita ignore membukit plak dia jadinye...bak kata kawanku...aduh!...sejenis nale betul!...tau apa tu nale? ntah saya pn xtau. tanyela pada kawan saya tu.dah lama tunggu dia singgah2 ke blog ni..tapi batang idung pn tak nampak. disebabkan tak ramai yg buka blog ni, so i have my right to write anything...huahuahua..eh! boleh ke?boleh jela...asalkan tak langgar undang2....

Jap lagi nak ambil adik kat johor,dah abis dah matrik die...hmmm...yg aku ni,tak abis2 lagi master,nak wat cane,nm lagi master....nak jd master kena pas byk peringkat,kalau tak pas kena repeat sampai lulus..yeke? taklah...tu master apa....harap2 hani dapat 4.0 mengalahkan diriku dulu..insyaAllah....

Petua

Bismillahirrahmanirrahimm...

Pagi tadi sms drive ke lab, saya ada terdengar satu petua untuk langsing at the same time jaga kesihatan. Seorang pendengar radio Hot.fm yg sampaikan petua ni...petuanya berbunyi begini:

Sebelum makan, ambil sebiji epal. tak kisah epal hijau ke epal merah...yg penting epal. Dan yang paling penting kita kena habiskan sebiji epal tu sebelum menjamah nasi. Then, barula makan nasi. Buah2 lain saya tak pasti kesannya sama ke tidak. Bila diamalkan selalu ia dikatakan dapat membuatkan kita cepat kenyang dan lama kelamaan dapat mengurangkan jumlah nasi yg kita ambil..

Petua ni baik untuk org2 yg nak kurangkan berat badan dan org2 berumur yg ingin mengurangkan pengambilan nasi berlebihan. kenapa saya kata org yg berumur (a.k.a org tua)? sebab saya pernah terdengar dari ramai org, org2 yg dah berumur kena kurangkan makanan yg tinggi carbohydrate.

Tiba2 saya terfikir...mungkinkah disebabkan kandungan serat epal atau nutrient lain yg terkandung didalamnya membuatkan kita cepat kenyang?? mungkin!..tapi apakah dia?...dalam masa yg sama, saya terbayang makanan2 "sihat" seperti oat dan sewaktu dgnnya selalu dicampurkan dgn epal...apa yg terbayang difikiran ialah..biskut Jacobs kat rumah -campuran oat & epal (sedap! =))dan satu objektif untuk harini bertambah. iaitu, apa yg menyebabkan epal selau digandingkan dgn makanan "melangsingkan" badan?apakah khasiatnya?...jom kita cari!!

Ops! belum habis lagi.....
Selepas makan nasi kita digalakkan menjarakkan masa minum air, dan kalau tak tahan ambil sepotong betik. Menjarakkan meminum air tadi adalah untuk menberi peluang kepada enzim2 dalam badan kita bertindak aktif, ini kerana dengan kehadiran air yg akan mencairkan (dilute) kuantiti enzim, kereaktifan enzim untuk bertindak balas akan berkurang. Sebab tu makanan lambat dihadam...... (emm...macam pelik plak kalau term2 ni ditulis dalam BM...hikhik)

okey, selamat mencuba!! (^_^)V